Full Services Available
Respirometry
Extracellular Flux Analyzers are available for respirometry assays in intact cells and spheroids, permeabilized cells, and isolated mitochondria. These assays can be used to quantify a wide range of mitochondrial bioenergetics parameters and are used to decipher the mechanism of mitochondrial bioenergitic function in a 96 well format in real time.
Imaging
High content imaging as well as high-resolution imaging is available for measurements of mitochondrial morphology and membrane potential, reactive oxygen species, mitophagy, NAD/NADH, cristae imaging, pH measurements, cell-monolayer electron microscopy, cell growth and differentiation and more. Additionally, use of equipment can be scheduled, and training is provided.
Biochemistry
Biochemical assays are available for detailed characterization of mitochondrial components, respiratory complexes, and mitochondria‑related pathways. Services include Western blotting for analysis of OXPHOS proteins, mitochondrial mass markers, fusion–fission dynamics, autophagy and mitophagy components, and lysosomal pathways, as well as Blue Native electrophoresis for assessment of OXPHOS and MICOS supercomplexes by immunoblotting and in‑gel activity measurements. Additional capabilities include plate-reader–based enzymatic assays such as lactate production, ATP levels, and other specific metabolic readouts, targeted gene expression analysis via qPCR, and mtDNAcopy number determination. Training, assay design support, and data interpretation are provided.
Consultation
Experts in a vast range of topics related to mitochondrial function are available to provide consulting related to study design, troubleshooting technical problems, method development, and data interpretation.
Training
Training is routinely provided for use of both the Extracellular Flux Analyzers and the High Content Imaging System. Additionally, guidance on assay design, data analysis and interpretation is provided for users.
